Composite

Part:BBa_K3765012:Design

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)


PelB_Signal+10xHis+SpyCatcher002+dCBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 634
    Illegal XhoI site found at 276
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 106
    Illegal AgeI site found at 472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 361


Design Notes

A pair of AgeI sites added before and after the SpyCatcher002 module to remove it if needed.


Source

Synthetic.

References